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Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci Genet. Mol. Biol.
Mantellatto,Aline Meira Bonfim; Caparroz,Renato; Christofoletti,Maurício Durante; Piovezan,Ubiratan; Duarte,José Maurício Barbanti.
Abstract The pampas deer (Ozotoceros bezoarticus) is close to being classified as ‘globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Fecal DNA; Microsatellites; Non-invasive methods; Pampas deer.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500774
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Molecular identification of Mazama species (Cervidae: Artiodactyla) from natural history collections Genet. Mol. Biol.
Mantellatto,Aline Meira Bonfim; González,Susana; Duarte,José Maurício Barbanti.
Abstract Natural history museum collections constitute an invaluable patrimony of biological diversity for analysing the taxa distribution and evolution. However, it is very common to discover taxonomic misidentification in museum collections based on incorrect data. The aim of this research was to identify brocket deer species (Mazama genus) using molecular markers. We collected 199 samples, performed DNA extraction and species identification using a specific mitochondrial marker based on a fragment of cytochrome b (Cytb) for Neotropical deer. We achieved the amplification and sequencing of 77 specimens and verified that 26% of the skulls were wrongly identified. Moreover, in the museum collections 57% of the specimens were only identified as Mazama sp,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytochrome b; Deer; DNA; Mammal; Morphological taxonomy.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400201
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